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e2f1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc e2f1
    (A, B) Kaplan–Meier plot showing that overexpression of <t>E2F1</t> is associated with shorter overall survival (A) and progression-free interval (B) in endometrial cancer cohort. (C, D) Kaplan–Meier plot showing that co-overexpression of LARP1 and E2F1 is associated with shorter overall survival (C) and progression-free interval (D) in endometrial cancer cohort. (E) Spearman correlation analysis shows a correlation between LARP1 and E2F1 in endometrial cancer patients.
    E2f1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 681 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2f1/E2F-1+Antibody/bio_rxiv__64898__2026__03__22__713473-71-26-32
    Average 96 stars, based on 681 article reviews
    e2f1 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "“Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”"

    Article Title: “Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”

    Journal: bioRxiv

    doi: 10.64898/2026.03.22.713473

    (A, B) Kaplan–Meier plot showing that overexpression of E2F1 is associated with shorter overall survival (A) and progression-free interval (B) in endometrial cancer cohort. (C, D) Kaplan–Meier plot showing that co-overexpression of LARP1 and E2F1 is associated with shorter overall survival (C) and progression-free interval (D) in endometrial cancer cohort. (E) Spearman correlation analysis shows a correlation between LARP1 and E2F1 in endometrial cancer patients.
    Figure Legend Snippet: (A, B) Kaplan–Meier plot showing that overexpression of E2F1 is associated with shorter overall survival (A) and progression-free interval (B) in endometrial cancer cohort. (C, D) Kaplan–Meier plot showing that co-overexpression of LARP1 and E2F1 is associated with shorter overall survival (C) and progression-free interval (D) in endometrial cancer cohort. (E) Spearman correlation analysis shows a correlation between LARP1 and E2F1 in endometrial cancer patients.

    Techniques Used: Over Expression

    (A, B) Immunoblot analysis showing the protein expression of LARP1 and E2F1 after transfecting ISHI (A) and HEC-1A (B) cells with control or LARP1 siRNA. β-actin was used as a loading control. (C) Images representing immunofluorescence staining of LARP1 (green) and E2F1 (red) in HEC-1A cells after transfection with control or LARP1 siRNA. DAPI was used as a counter stain. Scale bar = 50 µm.
    Figure Legend Snippet: (A, B) Immunoblot analysis showing the protein expression of LARP1 and E2F1 after transfecting ISHI (A) and HEC-1A (B) cells with control or LARP1 siRNA. β-actin was used as a loading control. (C) Images representing immunofluorescence staining of LARP1 (green) and E2F1 (red) in HEC-1A cells after transfection with control or LARP1 siRNA. DAPI was used as a counter stain. Scale bar = 50 µm.

    Techniques Used: Western Blot, Expressing, Control, Immunofluorescence, Staining, Transfection

    Related Articles

    other:

    Article Title: ATAD2 drives melanoma growth and progression and inhibits ferroptosis
    Article Snippet: E2F1 , Cell Signaling , Cat# 3742.

    Western Blot:

    Article Title: Separate transcription and splicing gene networks are linked and coordinated by the pRb–E2F pathway
    Article Snippet: .. The following antibodies were used in immunoblots: β-actin (clone AC-74, Sigma–Aldrich, St. Louis, USA), E2F1 (3742S, Cell Signaling Technology, Danvers, USA), GAPDH (clone 6C5, MAB374, Millipore, Burlington, USA), HNRNPC1/C2 (clone 4F4, sc-32308, Santa Cruz, Dallas, USA), SC35 (EPR12238, ab204916, Abcam, Cambridge, UK), SRSF2 (PA5-12402, Thermo Fisher, Waltham, USA), pRb (clone 4H1, 9309S, Cell Signaling Technology, Danvers, USA), DP-1 (clone TFD-10, sc-53642, Santa Cruz, Dallas, USA), and symmetric di-methyl arginine (SDMe) (13222S, Cell Signaling Technology, Danvers, USA). ..

    Article Title: Sensitizing tumor response to topoisomerase I antibody drug conjugate by selective CDK7 inhibition
    Article Snippet: Trastuzumab deruxtecan (T-DXd) and sacituzumab govitecan (SG) were purchased from Evidentic GmbH (Potsdam, Germany). .. The following antibodies were used for immunoblotting: total CDK7 (Cell Signaling Technology, Danvers, MA, USA, 2090S, RRID: AB_2077140), MYC (Cell Signaling Technology, 13987, RRID: AB_2631168), E2F1 (Cell Signaling Technology, 3742, RRID: AB_2096936), MYC S62P (Cell Signaling Technology, 13748, RRID: AB_2687518), MYC T58P (Cell Signaling Technology, 46650), Rb S795P (Cell Signaling Technology, 9301, RRID: AB_330013), Rb S780P (Cell Signaling Technology, 9307, RRID: AB_330015), MAX (Cell Signaling Technology, 4739, RRID: AB_2281777), T-H2AX (Santa Cruz, Dallas, TX, USA, sc-517336, RRID: AB_3675923), P-H2AX (Cell Signaling Technology, 2577, RRID: AB_2118010), Ubiquitin (Santa Cruz, sc-8017, RRID: AB_628423), TOP1 (BD Biosciences, San Jose, CA, USA, 556597, RRID: AB_396474), β-actin (Santa Cruz, sc-47778, RRID: AB_626632), Affinity Purified Goat Anti-Rabbit IgG (H+L)-HRP (Bio-Rad, Hercules, CA, USA, 1706515, RRID: AB_11125142), and Affinity Purified Goat Anti-Mouse IgG (H+L)-HRP (Bio-Rad, 1706516, RRID: AB_11125547). .. The following antibodies were used for ChIP-seq: pan pol II (Cell Signaling Technology, 2629 RRID: AB_2167468), Ser5P (Abcam, Cambridge, UK, ab5408, RRID:AB_304868), Ser2P (Abcam, ab5095, RRID:AB_304749), CDK7 (Bethyl, Montgomery, TX, USA, A300-405A, RRID: AB_2275973), MYC (Cell Signaling Technology, 13987, RRID: AB_2631168), E2F1 (Cell Signaling Technology, 3742, RRID: AB_2096936), and TOP1 (Abcam, ab109374, RRID: AB_10861978).

    Ubiquitin Proteomics:

    Article Title: Sensitizing tumor response to topoisomerase I antibody drug conjugate by selective CDK7 inhibition
    Article Snippet: Trastuzumab deruxtecan (T-DXd) and sacituzumab govitecan (SG) were purchased from Evidentic GmbH (Potsdam, Germany). .. The following antibodies were used for immunoblotting: total CDK7 (Cell Signaling Technology, Danvers, MA, USA, 2090S, RRID: AB_2077140), MYC (Cell Signaling Technology, 13987, RRID: AB_2631168), E2F1 (Cell Signaling Technology, 3742, RRID: AB_2096936), MYC S62P (Cell Signaling Technology, 13748, RRID: AB_2687518), MYC T58P (Cell Signaling Technology, 46650), Rb S795P (Cell Signaling Technology, 9301, RRID: AB_330013), Rb S780P (Cell Signaling Technology, 9307, RRID: AB_330015), MAX (Cell Signaling Technology, 4739, RRID: AB_2281777), T-H2AX (Santa Cruz, Dallas, TX, USA, sc-517336, RRID: AB_3675923), P-H2AX (Cell Signaling Technology, 2577, RRID: AB_2118010), Ubiquitin (Santa Cruz, sc-8017, RRID: AB_628423), TOP1 (BD Biosciences, San Jose, CA, USA, 556597, RRID: AB_396474), β-actin (Santa Cruz, sc-47778, RRID: AB_626632), Affinity Purified Goat Anti-Rabbit IgG (H+L)-HRP (Bio-Rad, Hercules, CA, USA, 1706515, RRID: AB_11125142), and Affinity Purified Goat Anti-Mouse IgG (H+L)-HRP (Bio-Rad, 1706516, RRID: AB_11125547). .. The following antibodies were used for ChIP-seq: pan pol II (Cell Signaling Technology, 2629 RRID: AB_2167468), Ser5P (Abcam, Cambridge, UK, ab5408, RRID:AB_304868), Ser2P (Abcam, ab5095, RRID:AB_304749), CDK7 (Bethyl, Montgomery, TX, USA, A300-405A, RRID: AB_2275973), MYC (Cell Signaling Technology, 13987, RRID: AB_2631168), E2F1 (Cell Signaling Technology, 3742, RRID: AB_2096936), and TOP1 (Abcam, ab109374, RRID: AB_10861978).

    Affinity Purification:

    Article Title: Sensitizing tumor response to topoisomerase I antibody drug conjugate by selective CDK7 inhibition
    Article Snippet: Trastuzumab deruxtecan (T-DXd) and sacituzumab govitecan (SG) were purchased from Evidentic GmbH (Potsdam, Germany). .. The following antibodies were used for immunoblotting: total CDK7 (Cell Signaling Technology, Danvers, MA, USA, 2090S, RRID: AB_2077140), MYC (Cell Signaling Technology, 13987, RRID: AB_2631168), E2F1 (Cell Signaling Technology, 3742, RRID: AB_2096936), MYC S62P (Cell Signaling Technology, 13748, RRID: AB_2687518), MYC T58P (Cell Signaling Technology, 46650), Rb S795P (Cell Signaling Technology, 9301, RRID: AB_330013), Rb S780P (Cell Signaling Technology, 9307, RRID: AB_330015), MAX (Cell Signaling Technology, 4739, RRID: AB_2281777), T-H2AX (Santa Cruz, Dallas, TX, USA, sc-517336, RRID: AB_3675923), P-H2AX (Cell Signaling Technology, 2577, RRID: AB_2118010), Ubiquitin (Santa Cruz, sc-8017, RRID: AB_628423), TOP1 (BD Biosciences, San Jose, CA, USA, 556597, RRID: AB_396474), β-actin (Santa Cruz, sc-47778, RRID: AB_626632), Affinity Purified Goat Anti-Rabbit IgG (H+L)-HRP (Bio-Rad, Hercules, CA, USA, 1706515, RRID: AB_11125142), and Affinity Purified Goat Anti-Mouse IgG (H+L)-HRP (Bio-Rad, 1706516, RRID: AB_11125547). .. The following antibodies were used for ChIP-seq: pan pol II (Cell Signaling Technology, 2629 RRID: AB_2167468), Ser5P (Abcam, Cambridge, UK, ab5408, RRID:AB_304868), Ser2P (Abcam, ab5095, RRID:AB_304749), CDK7 (Bethyl, Montgomery, TX, USA, A300-405A, RRID: AB_2275973), MYC (Cell Signaling Technology, 13987, RRID: AB_2631168), E2F1 (Cell Signaling Technology, 3742, RRID: AB_2096936), and TOP1 (Abcam, ab109374, RRID: AB_10861978).

    Blocking Assay:

    Article Title: Cuproptosis-associated PDHA1 promotes sarcoma progression and immunotherapy responsiveness via the E2F1-PD-L1 axis: a multi-omics and clinical validation study.
    Article Snippet: .. After blocking with 5% non-fat milk for 1 h at room temperature, membranes were incubated overnight at 4 °C with primary antibodies against PDHA1 (ab168379, Abcam), E2F1 (#3742, CST), PD-L1 (#13684, CST), Bcl-2 (ab182858, Abcam), MMP-9 (ab76003, Abcam), cleaved Caspase-3 (#9661, CST), and lipoylated DLAT (anti-lipoic acid rabbit pAb, 437695, Millipore), as well as GAPDH (ab8245, Abcam; 1:2,000) as a loading control. .. After incubation with HRP-conjugated secondary antibodies for 1 h at room temperature, protein bands were visualized using enhanced chemiluminescence reagents (ECL, Millipore) and quantified using ImageJ software.

    Incubation:

    Article Title: Cuproptosis-associated PDHA1 promotes sarcoma progression and immunotherapy responsiveness via the E2F1-PD-L1 axis: a multi-omics and clinical validation study.
    Article Snippet: .. After blocking with 5% non-fat milk for 1 h at room temperature, membranes were incubated overnight at 4 °C with primary antibodies against PDHA1 (ab168379, Abcam), E2F1 (#3742, CST), PD-L1 (#13684, CST), Bcl-2 (ab182858, Abcam), MMP-9 (ab76003, Abcam), cleaved Caspase-3 (#9661, CST), and lipoylated DLAT (anti-lipoic acid rabbit pAb, 437695, Millipore), as well as GAPDH (ab8245, Abcam; 1:2,000) as a loading control. .. After incubation with HRP-conjugated secondary antibodies for 1 h at room temperature, protein bands were visualized using enhanced chemiluminescence reagents (ECL, Millipore) and quantified using ImageJ software.

    Article Title: “Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”
    Article Snippet: .. Cells were then incubated overnight at 4 °C with the following primary antibodies: LARP1 (Cat. No. sc-515873; 1:200 dilution; Santa Cruz Biotechnology, Dallas, TX, USA) and E2F1 (Cat. No. 3742S; 1:400 dilution; Cell Signaling Technology, Danvers, MA, USA). .. After washing with PBS, cells were incubated for 1 h at room temperature with secondary antibodies: goat anti-mouse IgG Alexa Fluor 488 (Cat. No. A-11001) and goat anti-rabbit IgG Alexa Fluor 568 (Cat. No. A-11011) (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA).

    Control:

    Article Title: Cuproptosis-associated PDHA1 promotes sarcoma progression and immunotherapy responsiveness via the E2F1-PD-L1 axis: a multi-omics and clinical validation study.
    Article Snippet: .. After blocking with 5% non-fat milk for 1 h at room temperature, membranes were incubated overnight at 4 °C with primary antibodies against PDHA1 (ab168379, Abcam), E2F1 (#3742, CST), PD-L1 (#13684, CST), Bcl-2 (ab182858, Abcam), MMP-9 (ab76003, Abcam), cleaved Caspase-3 (#9661, CST), and lipoylated DLAT (anti-lipoic acid rabbit pAb, 437695, Millipore), as well as GAPDH (ab8245, Abcam; 1:2,000) as a loading control. .. After incubation with HRP-conjugated secondary antibodies for 1 h at room temperature, protein bands were visualized using enhanced chemiluminescence reagents (ECL, Millipore) and quantified using ImageJ software.

    Saline:

    Article Title: The Small Molecule SR8278 Inhibits Cell Proliferation Independent of the REV-ERB Nuclear Receptor Proteins in Human Keratinocytes
    Article Snippet: Blots were stained with 0.5% Ponceau S (Sigma P3504, St. Louis, MO, USA) to ensure equal loading. .. The blots were blocked in 5% non-fat milk (Meijer, Tipp City, OH, USA) in TBST (Tris-buffered saline containing 0.1% Tween-20) and then probed overnight with primary antibodies from Cell Signaling Technology (Danvers, MA, USA) recognizing E2F1 (#3742), RRM2 (#65939), Cyclin E2 (#4132), phospho-CHK1 (Ser345; #2348), phospho-H2AX (Ser1139; #9718), PARP (#9542), or REV-ERBα (#13418), antibodies from Santa Cruz Biotechnology (Dallas, TX, USA) recognizing PCNA (sc-56) or REV-ERBβ (sc-398252), or an antibody from Bethyl recognizing phospho-KAP1 (Ser824; A300-767A). .. After washing with TBST, blots were probed with HRP-coupled anti-rabbit or anti-mouse IgG (Invitrogen) secondary antibodies for one hour at room temperature.



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    Image Search Results


    E2F1 transcriptionally activates TRIP13 to drive Palbociclib resistance in prostate cancer. In TCGA prostate cancer, samples were stratified into high and low TRIP13 expression groups. Differential expression analysis was performed, followed by GSEA enrichment analysis. In the bubble plot, different colors represent NES values, while bubble sizes correspond to the log-adjusted P -values (A) . Spearman correlation plots showing the relationship between E2F1 and TRIP13, panels: (B) TCGA prostate cancer data, (C) PRAD_SU2C_2019 data. (D) Ominer network tool prediction of potential transcription factors for TRIP13. (E) Chip-Seq analysis of E2F1 binding to the TRIP13 promoter region from Chip-Atlas. (F) GPSAdb data indicate reduced TRIP13 expression following E2F1 knockdown. Different colors represent different LogFC. (G) PC-3 cells were transfected with specified shRNA for 72 hours, followed by puromycin selection. These cells were treated with collected for ChIP-qPCR analysis using IgG or E2F1 antibodies. Data represent the mean ± SEM from three independent experiments. ** indicates P < 0.01, *** indicates P < 0.001. (H, I) PC-3 and C4-2 cells were transfected with specified shRNA for 72 hours. After puromycin screening, cells were subjected to western blot analysis (H) and RT-qPCR assay (I) . Data represent the mean ± SEM from three independent experiments. ** indicates P < 0.01, *** indicates P < 0.001. (J, K) PC-3 and C4-2 cells were transfected with designated plasmids for 24 hours. Following puromycin screening, cells underwent western blot analysis (J) and RT-qPCR assay (K) . Data are presented as the mean ± SEM from three independent experiments. *** indicates P < 0.001. (L) The sequence and location of the E2F1 binding peak in the TRIP13 promoter. WT, wild type, MUT, mutant. (M) PC-3 cells were transfected with indicated plasmid for 24 h. After puromycin selection, the cells were harvested and the activity of the TRIP13 promoters was measured. Data are presented as the mean ± SEM from three independent experiments. ** indicates P < 0.01, ns, not significant. (N) PC-3 and C4-2 cells were transfected with specified shRNA for 72 hours and indicated plasmid for 24 h, followed by puromycin selection. The cells were then treated with incremental doses of palbociclib for 24 hours and subjected to CCK-8 assays. Data were expressed as the mean ± SEM and repeated three times. Statistical significance was determined using the indicated tests, with P <0.05 considered significant.

    Journal: Frontiers in Immunology

    Article Title: Multi-omics analysis and functional validation reveal the oncogenic role of TRIP13

    doi: 10.3389/fimmu.2026.1691436

    Figure Lengend Snippet: E2F1 transcriptionally activates TRIP13 to drive Palbociclib resistance in prostate cancer. In TCGA prostate cancer, samples were stratified into high and low TRIP13 expression groups. Differential expression analysis was performed, followed by GSEA enrichment analysis. In the bubble plot, different colors represent NES values, while bubble sizes correspond to the log-adjusted P -values (A) . Spearman correlation plots showing the relationship between E2F1 and TRIP13, panels: (B) TCGA prostate cancer data, (C) PRAD_SU2C_2019 data. (D) Ominer network tool prediction of potential transcription factors for TRIP13. (E) Chip-Seq analysis of E2F1 binding to the TRIP13 promoter region from Chip-Atlas. (F) GPSAdb data indicate reduced TRIP13 expression following E2F1 knockdown. Different colors represent different LogFC. (G) PC-3 cells were transfected with specified shRNA for 72 hours, followed by puromycin selection. These cells were treated with collected for ChIP-qPCR analysis using IgG or E2F1 antibodies. Data represent the mean ± SEM from three independent experiments. ** indicates P < 0.01, *** indicates P < 0.001. (H, I) PC-3 and C4-2 cells were transfected with specified shRNA for 72 hours. After puromycin screening, cells were subjected to western blot analysis (H) and RT-qPCR assay (I) . Data represent the mean ± SEM from three independent experiments. ** indicates P < 0.01, *** indicates P < 0.001. (J, K) PC-3 and C4-2 cells were transfected with designated plasmids for 24 hours. Following puromycin screening, cells underwent western blot analysis (J) and RT-qPCR assay (K) . Data are presented as the mean ± SEM from three independent experiments. *** indicates P < 0.001. (L) The sequence and location of the E2F1 binding peak in the TRIP13 promoter. WT, wild type, MUT, mutant. (M) PC-3 cells were transfected with indicated plasmid for 24 h. After puromycin selection, the cells were harvested and the activity of the TRIP13 promoters was measured. Data are presented as the mean ± SEM from three independent experiments. ** indicates P < 0.01, ns, not significant. (N) PC-3 and C4-2 cells were transfected with specified shRNA for 72 hours and indicated plasmid for 24 h, followed by puromycin selection. The cells were then treated with incremental doses of palbociclib for 24 hours and subjected to CCK-8 assays. Data were expressed as the mean ± SEM and repeated three times. Statistical significance was determined using the indicated tests, with P <0.05 considered significant.

    Article Snippet: OmicLinkTM Expression Clone (CMV Promoter) plasmids (Catalog #: EX-V0006-M14, GeneCopoeia, USA) were utilized to clone the cDNA of TRIP13 and E2F1 for their overexpression.

    Techniques: Expressing, Quantitative Proteomics, ChIP-sequencing, Binding Assay, Knockdown, Transfection, shRNA, Selection, ChIP-qPCR, Western Blot, Quantitative RT-PCR, Sequencing, Mutagenesis, Plasmid Preparation, Activity Assay, CCK-8 Assay

    (A, B) Kaplan–Meier plot showing that overexpression of E2F1 is associated with shorter overall survival (A) and progression-free interval (B) in endometrial cancer cohort. (C, D) Kaplan–Meier plot showing that co-overexpression of LARP1 and E2F1 is associated with shorter overall survival (C) and progression-free interval (D) in endometrial cancer cohort. (E) Spearman correlation analysis shows a correlation between LARP1 and E2F1 in endometrial cancer patients.

    Journal: bioRxiv

    Article Title: “Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”

    doi: 10.64898/2026.03.22.713473

    Figure Lengend Snippet: (A, B) Kaplan–Meier plot showing that overexpression of E2F1 is associated with shorter overall survival (A) and progression-free interval (B) in endometrial cancer cohort. (C, D) Kaplan–Meier plot showing that co-overexpression of LARP1 and E2F1 is associated with shorter overall survival (C) and progression-free interval (D) in endometrial cancer cohort. (E) Spearman correlation analysis shows a correlation between LARP1 and E2F1 in endometrial cancer patients.

    Article Snippet: Cells were then incubated overnight at 4 °C with the following primary antibodies: LARP1 (Cat. No. sc-515873; 1:200 dilution; Santa Cruz Biotechnology, Dallas, TX, USA) and E2F1 (Cat. No. 3742S; 1:400 dilution; Cell Signaling Technology, Danvers, MA, USA).

    Techniques: Over Expression

    (A, B) Immunoblot analysis showing the protein expression of LARP1 and E2F1 after transfecting ISHI (A) and HEC-1A (B) cells with control or LARP1 siRNA. β-actin was used as a loading control. (C) Images representing immunofluorescence staining of LARP1 (green) and E2F1 (red) in HEC-1A cells after transfection with control or LARP1 siRNA. DAPI was used as a counter stain. Scale bar = 50 µm.

    Journal: bioRxiv

    Article Title: “Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”

    doi: 10.64898/2026.03.22.713473

    Figure Lengend Snippet: (A, B) Immunoblot analysis showing the protein expression of LARP1 and E2F1 after transfecting ISHI (A) and HEC-1A (B) cells with control or LARP1 siRNA. β-actin was used as a loading control. (C) Images representing immunofluorescence staining of LARP1 (green) and E2F1 (red) in HEC-1A cells after transfection with control or LARP1 siRNA. DAPI was used as a counter stain. Scale bar = 50 µm.

    Article Snippet: Cells were then incubated overnight at 4 °C with the following primary antibodies: LARP1 (Cat. No. sc-515873; 1:200 dilution; Santa Cruz Biotechnology, Dallas, TX, USA) and E2F1 (Cat. No. 3742S; 1:400 dilution; Cell Signaling Technology, Danvers, MA, USA).

    Techniques: Western Blot, Expressing, Control, Immunofluorescence, Staining, Transfection